Education

• Ph.D., Pharmaceutical Sciences (University of Florida, 1984)
• M.S., Pathology (University of Florida, 1980)
• B.S., Chemistry (Illinois State University, 1977)
• A.A.S, Medical Technology (Illinois Central College, 1974)

Academic Appointments/Professional Experience

• (2005 - Present) Fellow, VCU Center for the Study of Biological Complexity
• (2004 - Present) Affiliate Professor, Department of Chemistry, Virginia Commonwealth University
• (1998 - Present) Affiliate Professor, Department of Medicinal Chemistry, Virginia Commonwealth University
• (1997 - 2000) Associate Dean for Research and Graduate Studies, School of Pharmacy, Virginia Commonwealth University
• (1996 - Present) Professor, Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University
• (1995 - Present) Director of Analytical Core Laboratories, Center for Drug Studies, School of Pharmacy, Virginia Commonwealth University
• (1992 - Present) Affiliate Professor, Biomedical Engineering, Virginia Commonwealth University
• (1990 - 1995) Director of Analytical laboratories, BioClin, Inc.
• (1984 - 1990) Director of Analytical Laboratories, Biopharm Center for Clinical Research, Virginia Commonwealth University
• (1984 - 1989) Assistant Professor, Department of Pharmacy and Pharmaceutics, School of Pharmacy, Virginia Commonwealth University
• (1981 - 1982) Research Chemist, Department of Pharmacy Practice, University of Florida, Gainesville, FL
• (1980 - 1981) Analytical Chemist, Analytical Toxicology Laboratory, Shands Teaching Hospital, Gainesville, FL

Professional and Scholarly Interests

• Bioanalysis using novel luminescence
• Mass spectroscopy, immunoreactor and laser spectroscopy systems
• Drug metabolism, drug release and biological drug distribution

Research Interests

• The research of our group involves the analytical chemistry of biological systems. We believe we are one of the most active groups of this kind in the World. Our work includes investigation of novel instrumental and chemical approaches to provide improved selectivity and sensitivity for quantitative analysis of drugs in biological samples. In past research, we have employed separation approaches such as capillary electrophoresis, and capillary electrochromatography; validation and control approaches for analytical methods; laser induced fluorescence, chemical derivatization to improve detection; chemiluminescence detection in liquid chromatography; on-line immunoreaction detectors and far-red solution spectroscopy. The research laboratory works in conjunction with a well equipped, state of the art bioanalytical core facility that provides students with "real world" experience and a steady source of research funding.

The publications search is performed on the National Library of Medicine's PubMed database. The search may be slow during high traffic hours, may return articles authored by another researcher with a similar name and may not always find all of a researcher's articles.

Publications

Books/Book Chapters

• Karnes HT: “Validation and Control of Bioanalytical Methods,” In: Figg WD, McLeod H, editors. Handbook of Anti-Cancer Pharmacokinetics and Pharmacodynamics–Cancer Drug Discovery and Development Series, New Jersey: Humana Press; 91-111, 2004.
Recent Publications

• Peoples MC and Karnes HT: “Microfluidic Immunoaffinity Separations for Bioanalysis”, J Chromatography B, 866(1-2): 14-25, 2008.
• Peoples MC and Karnes HT: “Microfluidic Capillary System for Immunoaffinity Separations of C-Reactive Protein in Human Serum and Cerebrospinal Fluid”, Analytical Chemistry 80: 3853-3858, 2008.
• Bishoff R, Hopfgartner G, Karnes HT, Lloyd DK, Phillips TM, Tsikas D and Xu G: “Summary of a Recent Workshop/Conference Report on Validation and Implementation of Bioanalytical Methods: Implications on Manuscript Review in the Journal of Chromatography B”, J Chromatogr. B, 860: 1-3, 2007.
• Iyer SS, Kellogg GE and Karnes HT: “A LC–Electrospray Tandem MS Method for the Analysis of Naltrexone in Canine Plasma Employing a Molecular Model to Demonstrate the Absence of Internal Standard Deuterium Isotope Effects”, J Chromatogr Sci., 45(10): 694-700, 2007.
• Farthing D, Gehr L, Karnes HT, Sica D, Gehr T, Larus T, Farthing C and Xe L: “Effects of Salicylic Acid on Post-ischemic Ventricular Function and Purine Efflux in Isolated Mouse Hearts”, Biomarkers, 12:6, 623-634, 2007.
• Ismaiel OA, Halquist MS, Elmamly MY, Shalaby AA and Karnes HT: “Monitoring Phospholipids for Assessment of Matrix Effects in a Liquid Chromatography-Tandem Mass Spectrometry Method for Hydrocodone and Pseudoephedrine in Human Plasma”, J Chromatography B, 859: 84-93, 2007.
• Martinez MN, Bonate P, Chapman RL, De Groot A, D’Souza S, Ghilzai N, Gray V, Gupta VK, Huynh-Ba K, Iyer SS, Jayatilaka A, Joshi A, Karnes HT, Khan M, Liu P, Lunte C, McCurdy CR, Morris ME, Norris K, Ramsey P, Sehgal S and Zahn M: “Highlights of Advances in the Pharmaceutical Sciences: An American Association of Pharmaceutical Scientists (AAPS) Perspective”, AAPS Journal, 9:2, E219-E226, 2007.
• Iyer SS, Barr WH and Karnes HT: “A Biorelevant Approach to Accelerated In Vitro Release Testing Study of a Biodegradable Naltrexone Implant”, Int J Pharm., 340: 119-125, 2007.
• Iyer SS, Barr WH, Dance ME, Coleman PR and Karnes HT: “A ‘Biorelevant’ System to Investigate In Vitro Drug Released from a Naltrexone Implant”, Int J Pharm., 340: 104-118, 2007.